Dual-genome primer design for construction of DNA microarrays

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Dual-genome primer design for construction of DNA microarrays

MOTIVATION Microarray experiments using probes covering a whole transcriptome are expensive to initiate, and a major part of the costs derives from synthesizing gene-specific PCR primers or hybridization probes. The high costs may force researchers to limit their studies to a single organism, although comparing gene expression in different species would yield valuable information. RESULTS We ...

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PrimeArray is a Windows program that computes oligonuceotide primer pairs for genome-scale gene amplification by the Polymerase Chain Reaction (PCR). The program supports the automated extraction of coding sequences (CDS) from various input-file formats and allows highly automated primer pair-optimization.

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OligoArray: genome-scale oligonucleotide design for microarrays

SUMMARY OligoArray is a program that computes gene specific and secondary structure free oligonucleotides for genome-scale oligonucleotide microarray construction or other applications. AVAILABILITY The program code is distributed under the GNU General Public License and is freely available for non-profit use via request from the authors.

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MOTIVATION The use of DNA microarrays allows the monitoring of the extreme microbial diversity encountered in complex samples like environmental ones as well as that of their functional capacities. However, no probe design software currently available is adapted to easily design efficient and explorative probes for functional gene arrays. RESULTS We present a new efficient functional microarr...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2004

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/bti001